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中国癌症防治杂志

中国癌症防治杂志 ›› 2013, Vol. 5 ›› Issue (4): 320-323.doi: 10.3969/j.issn.1674-5671.2013.04.08

• 临床研究 • 上一篇    下一篇

非小细胞肺癌患者血清IL-17和VEGF含量的检测及其临床意义

  

  1. 广西医科大学附属肿瘤医院化疗二科
  • 出版日期:2013-12-25 发布日期:2013-12-31
  • 通讯作者: 宋向群 E-mail:xingquns@163.com
  • 基金资助:

    广西自然科学基金资助项目（桂科自0991233）

Detection and clinical significance of serum levels of IL-17 and VEGF in patients with NSCLC

  • Online:2013-12-25 Published:2013-12-31

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摘要： 目的 检测非小细胞肺癌（non-small cell lung cancer，NSCLC）患者血清中白细胞介素17（interleukin-17，IL-17）和血管内皮生长因子（vascular endothelial growth factor，VEGF）的含量并分析其临床意义。方法 采用双抗体夹心酶联免疫吸附法（ABC-ELISA）检测52例 NSCLC患者术前血清和26名健康体检者血清中IL-17和VEGF的水平， 比较两组血清中IL-17和VEGF含量水平的差异，并分析其与临床病理特征的关系。结果 52例NSCLC血清中IL-17和VEGF的含量均明显高于26名健康志愿者（P =0.000）。NSCLC患者血清中IL-17和VEGF的含量呈负相关（r=-0.88，P =0.000）。NSCLC患者血清中IL-17的水平与吸烟状态、病理类型和临床分期相关，其中吸烟人群高于非吸烟人群（P =0.048），鳞癌高于腺癌（P =0.001），随着临床分期的增加，IL-17的水平逐渐降低。NSCLC患者血清中VEGF的水平与分化程度、临床分期相关，其中低分化患者高于中高分化者（P =0.000），且VEGF的水平随临床分期的增加而逐渐升高。结论 NSCLC患者血清中IL-17和VEGF的水平均升高，两者均与临床病理特征密切相关，检测血清中IL-17 和VEGF的水平对判断NSCLC患者的疾病状态有一定的参考价值。

关键词:  , 肺肿瘤, 非小细胞肺癌, 白细胞介素17, 血管内皮生长因子, 双抗体夹心酶联免疫吸附法

Abstract: Objective To detect serum levels of IL-17 and VEGF in patients with non-small cell lung cancer（NSCLC） and assess their clinical significance. Methods Serum levels of IL-17 and VEGF were determined in 52 NSCLC patients and 26 healthy controls using avidin-biotin complex ELISA.The levels as well as clinicopathological features were compared between the two groups. Results Serum levels of both IL-17 and VEGF levels were significantly higher in patients than in controls（P=0.000）.In NSCLC patients， serum IL-17 negatively correlated with VEGF expression （r=-0.88，P=0.000）.IL-17 expression was higher in smokers than non-smokers（P=0.048） and higher in squamous cell carcinoma patients than in adenocarcinoma patients（P=0.001）.IL-17 concentration decreased and VEGF concentration increased with increasing NSCLC grade.Serum VEGF level was significantly higher in patients with poorly differentiated tumors than in those with well-differentiated ones（P=0.000）. Conclusions Serum levels of IL-17 and VEGF were significantly elevated in NSCLC patients and were strongly associated with clinicopathological features. Therefore，assaying IL-17 and VEGF may be useful for evaluating the prognosis of NSCLC patients.

Key words:  Lung neoplasms, Non-small cell lung cancer, IL-17, VEGF, Avidin-biotin complex enzyme-linked immunosorbent assay